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歡迎光臨福州南江生物科技有限公司

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IPKine? GFP標簽蛋白免疫沉淀試劑盒（磁珠法）

貨號 品牌 CAS 規(guī)格 銷售價(RMB) 折扣價(RMB) 備注 數(shù)量
KTI2024-100T Abbkine 無 100T 3698 3698 無
KTI2024-20T Abbkine 無 20T 958 958 無
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商品信息

產品英文名稱IPKine? Anti-GFP Magnetic IP Kit
免疫原合成多肽
反應性Mammals, Bacteria
應用IP
實驗建議綠色熒光蛋白標簽（GFP-Tag，GFP標簽）小鼠單克隆抗體通過共價鍵偶聯(lián)磁珠，可用于免疫沉淀檢測和捕獲含有GFP-Tag序列的融合蛋白，優(yōu)化后的Anti-GFP Magnetic Beads具有更高效的抗原結合能力。根據目的蛋白的結構、生物學功能及后續(xù)應用的要求等，本試劑盒提供兩種洗脫方法，包括酸洗脫和SDS-PAGE Loading Buffer洗脫方法對目的蛋白進行洗脫。特別是酸洗脫后不會包含抗體的輕鏈和重鏈，可以有效解決免疫沉淀后Western Blotting實驗中抗體重鏈和輕鏈的干擾問題。
偶聯(lián)物磁珠

商品屬性

試劑盒組分
Non-Denaturing Lysis Buffer
TBS (10×)
Anti-GFP Magnetic Beads
Mouse IgG Magnetic Beads
Elution Buffer
Neutralization Buffer
SDS-PAGE Loading Buffer (5×)
特點&優(yōu)勢? 高效：特異性強、靶蛋白結合量高，≥0.6 mg GFP標簽融合蛋白/mL磁珠；
? 通用：提供IP實驗所需的所有必要緩沖液；
? 可靠：提供陰性對照，可排除IgG本身和目的蛋白或其它特定生物分子的非特異性結合；
? 靈活：本試劑盒提供兩種洗脫方法（酸洗脫和SDS-PAGE Loading Buffer洗脫方法）。
保存建議按各組分標簽提示分開存儲，保質期12個月。
運輸條件藍冰運輸
警告本產品僅供科學研究使用，不適用于臨床診斷。

附加信息

背景The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kD) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish. The GFP has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.

Figure. The immunoprecipitation effect of Anti-GFP Magnetic IP Kit used for GFP-Tag fusion protein. HEK293T cells were transfected with GFP-Tag plasmid. Lane 1 was whole cell lysate (WCL); Lane 2 was the immunoprecipitation sample of Mouse IgG Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer; Lane 3 was the immunoprecipitation sample of Anti-GFP Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer. Lane 4 was the immunoprecipitation sample of Anti-GFP Magnetic Beads eluted by Elution Buffer. By using acid elution, only contained GFP-Tag fusion protein, did not contain heavy and light chains of antibody.