貨號(hào) | 品牌 | CAS | 規(guī)格 | 銷售價(jià)(RMB) | 折扣價(jià)(RMB) | 備注 | 數(shù)量 |
KTI2034-100T | Abbkine | 無 | 100T | 3698 | 3698 | 無 | |
KTI2034-20T | Abbkine | 無 | 20T | 958 | 958 | 無 |
產(chǎn)品英文名稱 | IPKine? Anti-GST Magnetic IP Kit |
免疫原 | 合成多肽 |
反應(yīng)性 | Mammals, Bacteria |
應(yīng)用 | IP |
實(shí)驗(yàn)建議 | 谷胱甘肽巰基轉(zhuǎn)移酶標(biāo)簽(GST-Tag,GST標(biāo)簽)小鼠單克隆抗體通過共價(jià)鍵偶聯(lián)磁珠,可用于免疫沉淀檢測(cè)和捕獲含有GST-Tag序列的融合蛋白,優(yōu)化后的Anti-GST Magnetic Beads具有更高效的抗原結(jié)合能力。根據(jù)目的蛋白的結(jié)構(gòu)、生物學(xué)功能及后續(xù)應(yīng)用的要求等,本試劑盒提供兩種洗脫方法,包括酸洗脫和SDS-PAGE Loading Buffer洗脫方法對(duì)目的蛋白進(jìn)行洗脫。特別是酸洗脫后不會(huì)包含抗體的輕鏈和重鏈,可以有效解決免疫沉淀后Western Blotting實(shí)驗(yàn)中抗體重鏈和輕鏈的干擾問題。 |
偶聯(lián)物 | 磁珠 |
試劑盒組分 | Non-Denaturing Lysis Buffer TBS (10×) Anti-GST Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer SDS-PAGE Loading Buffer (5×) |
特點(diǎn)&優(yōu)勢(shì) | ? 高效:特異性強(qiáng)、靶蛋白結(jié)合量高,≥0.6 mg GST標(biāo)簽融合蛋白/mL磁珠; ? 通用:提供IP實(shí)驗(yàn)所需的所有必要緩沖液; ? 可靠:提供陰性對(duì)照,可排除IgG本身和目的蛋白或其它特定生物分子的非特異性結(jié)合; ? 靈活:本試劑盒提供兩種洗脫方法(酸洗脫和SDS-PAGE Loading Buffer洗脫方法)。 |
保存建議 | 按各組分標(biāo)簽提示分開存儲(chǔ),保質(zhì)期12個(gè)月。 |
運(yùn)輸條件 | 藍(lán)冰運(yùn)輸 |
警告 | 本產(chǎn)品僅供科學(xué)研究使用,不適用于臨床診斷。 |
背景 | The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial, and microsomal proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. GST tag antibody can be helpful in detecting the fusion protein during purification as well as the cleavage of GST from the protein of interest. GST tag antibody has wide applications that could include your research on GST proteins or GST fusion recombinant proteins. |
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